Bio-rad ChromLab™ Software Manual de usuario Pagina 249
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- Tabla de contenidos
- MARCADORES
Valorado. / 5. Basado en revisión del cliente
Evaluating Fractions
User Guide | 247
Tube location — the location of the group of pooled tubes in the rack.
Start —the beginning of the collection of the first fraction in the pool.
End — the end of the collection of the last fraction in the pool.
Collected volume (ml) — the total fraction volume collected in all tubes in
the pool.
Area (ml*mAU) — the sum of all areas in the pool. This column appears
after peak integration is performed.
Relative area (%) — the relative pool area of all areas in a trace. This
column appears after peak integration is performed.
Amount (mg) — the sum of all fraction amounts in the pool. This column
appears after peak integration is performed.
Concentration (mg/ml) — the calculated protein concentration for the
specified pool. This value is the weighted average by volume of the fraction
concentrations in the pool.
Molarity (M) — the calculated molar concentration of the protein for the
pool. This value is the weighted average by volume of the molar
concentrations for the fractions in the pool.
To create a fraction pool
1. In the Fractions table, hold down Ctrl or Shift and select multiple adjacent rows
of fractions.
2. Right-click the group of adjacent fractions and select Pool.
To expand the pool in the Fractions table
Do one of the following:
In the Fractions table, click the plus sign in the first cell.
Right-click the Fractions table and select Expand all pools.
To clear a pool
In the Fractions table, right-click the pool row and select Unpool.
- Chromatography Systems 1
- Software 1
- User Guide 1
- NGC™ Chromatography 3
- Systems and 3
- User Guide 7
- User Guide 9
- Table of Contents 10
- User Guide 11
- 1 Introduction 13
- Main NGC Features 14
- User Guide 15
- NGC Chromatography Systems 16
- User Guide 17
- Finding Out More 18
- 2 The Workspace 19
- The Home Window 20
- User Guide 21
- User Guide 23
- The System Control Window 25
- The Method Editor Window 26
- The Evaluation Window 27
- 2 28
- Touch Screen Menu Commands 29
- Touch Screen Toolbar Commands 30
- 3 System Control 31
- 3 32
- Manual Menu Commands 34
- Tools Menu Commands 34
- Context Menu Commands 35
- Chromatogram View 37
- Table 1. Trace Definitions 39
- Trace Module Explanation 40
- Showing or Hiding Traces 41
- Changing Trace Color 42
- Changing the Y-Scale Values 43
- Autoscaling the UV Trace 43
- To add annotations 45
- To edit an annotation 45
- To delete an annotation 45
- User Guide 47
- Fluidic Scheme Pane 48
- User Guide 49
- Sample Inject Valve 51
- Sample Pump 52
- Column Switching Valve 52
- UV/Conductivity Monitors 52
- Signal Import Module 52
- Fluidic Scheme Configurations 53
- User Guide 55
- User Guide 57
- Working with Fluidic Schemes 59
- To change module settings 61
- Fluidic Scheme Mapping 62
- Color Label valve 63
- To unmap valves 64
- To cancel unmapping valves 64
- To map valves 65
- Calibrations 66
- User Guide 67
- Calibrating Pressure Settings 68
- User Guide 69
- To verify the plumbing path 70
- System Settings 71
- To set the delay volume 72
- Orange 0.02" (0.5 mm) 73
- Green 0.03" (0.75 mm) 73
- Clear 0.062" (1.6 mm) 73
- Control Flow Tab 74
- To control the flow rate 75
- Remote Access Tab 76
- Trace Settings Tab 76
- User Guide 77
- Device Input Tab 78
- User Guide 79
- Device Output Tab 80
- To disable the connection 81
- Air Sensors Tab 82
- To deactivate an air sensor 83
- Email Notifications Tab 84
- User Guide 85
- System Name Tab 87
- System Information 88
- Detector Tab 89
- Multi-Wavelength Detector 90
- Preferences 91
- User Guide 93
- 4 Performing a Manual Run 95
- User Guide 97
- User Guide 99
- Preparing the System 101
- User Guide 101
- User Guide 103
- Cleaning the System 104
- Running an Experiment 105
- User Guide 107
- User Guide 109
- To run an experiment step 110
- Changing Module Settings 111
- Stopping a Manual Run 111
- Clearing Run Data 111
- Saving a Manual Run 111
- Viewing Run Data 113
- 5 Method Editor 115
- Before You Begin 116
- Opening a Method 117
- 5 118
- File Menu Commands 119
- Edit Menu Commands 120
- View Menu Commands 121
- Help Menu Commands 121
- Toolbar Commands 121
- Phase Library Pane 122
- Adding a Phase to a Method 123
- Method Outline Pane 124
- Phase Parameters Pane 124
- Method Steps Pane 125
- To view step details 126
- Method Settings Parameters 127
- Column Selection 128
- User Guide 129
- To add user-defined columns 131
- Detector Settings 133
- Unit Selection 133
- Fraction Collection 134
- Buffer Selection 135
- Pump Inlets 136
- Pump + Inlet Valves 136
- Pump + Buffer Blending 136
- To select a buffer 137
- Phase Controls and Parameters 138
- User Guide 139
- Standard Phases 140
- User Guide 141
- User Guide 143
- Column Wash Parameters 145
- Elution Parameters 146
- Special Considerations 147
- NGC Scout Systems 148
- User Guide 149
- User Guide 151
- To clean the pH valves 152
- User Guide 153
- User Guide 155
- System Preparation Parameters 156
- Create New Phase Parameters 157
- Step Library Pane 158
- Scout Parameters Pane 159
- 6 Creating a Method 161
- Standard Method Templates 162
- User Guide 163
- 6 164
- User Guide 165
- User Guide 167
- To create a method 168
- To change the fluidic scheme 168
- To specify general settings 169
- User Guide 171
- Adding Phases 172
- Editing Methods and Phases 172
- Renaming Phases 173
- Rearranging Phases 173
- Deleting Phases 174
- User Guide 175
- To run a method 177
- To add runs to the run queue 177
- Saving a Method 179
- Renaming a Method 180
- Deleting a Method 181
- The Scouting Wizard 183
- User Guide 185
- User Guide 187
- User Guide 189
- Creating a Scouting Method 191
- User Guide 193
- Running a Scouting Method 194
- To run a scout method 195
- 7 Evaluating Results 197
- Managing Analysis Projects 198
- To open a saved analysis 199
- 7 200
- Analysis Menu Commands 203
- Tab Toolbar Commands 204
- Customizing the Chromatogram 205
- Selecting the Active Trace 206
- Showing or Hiding a Trace 207
- Changing the Axes 207
- Changing Trace Colors 208
- Zooming In and Out 209
- Chromatogram 210
- To resize the selected region 211
- Annotating the Chromatogram 212
- Copying the Chromatogram 213
- Changing Table Grouping 214
- Sorting Table Columns 215
- Copying a Table 216
- User Guide 217
- Managing Runs 218
- To close a tab 219
- To close all tabs 219
- Comparing Traces 220
- Stacked View 221
- Overlay View 223
- Hiding All Traces in a Group 224
- Showing All Traces in a Group 224
- To offset a trace 225
- Managing Analyses 226
- Copying an Analysis 227
- Renaming an Analysis 227
- Exporting Run Data 228
- Peak Integration 229
- Baseline Parameters 230
- Peak Parameters 231
- Peak Filtering 231
- Default Parameters 231
- Starting Peak Integration 232
- User Guide 233
- Changing Peak View Options 234
- User Guide 235
- Showing or Hiding Columns 237
- Copying the Peaks Table 238
- User Guide 239
- Adjusting Peaks Manually 240
- To add a peak 241
- Saving Peak Integration Data 242
- Evaluating Fractions 243
- Rack Display 244
- Outlet Valve Display 244
- Fractions Table 245
- User Guide 247
- Viewing Fraction Details 248
- Pooling Fractions 248
- To create a fraction pool 249
- To clear a pool 249
- Viewing Heat Map Details 251
- To hide the rack display 252
- Column Performance Analysis 253
- Calculate 254
- Analyzing Column Performance 255
- Click Delete 256
- Column Performance Table 258
- User Guide 259
- 8 Importing and Exporting 261
- To import a method or run 262
- Importing Unicorn Data Files 263
- User Guide 265
- Exporting Data 266
- Exporting Data as an NGC File 267
- To export only the method 268
- To export only the run data 268
- User Guide 269
- To export multiple runs 270
- User Guide 271
- Exporting Diagnostic Logs 273
- Change the date range 274
- User Guide 275
- 9 Reports 277
- Producing a Report 278
- Method Reports 279
- Run Reports 279
- Printing a Report 280
- Saving a Report 281
- 9 282
- A Database Management 283
- Back Up the NGC Database 284
- Restore the NGC Database 285
- A 286
- B Multicolumn Purifications 287
- Method base unit 288
- Sample volume 288
- Step duration and length 288
- User Guide 289
- Template Name Description 291
- Plumbing the NGC System 293
- Purification Templates 294
- User Guide 295
- Priming the NGC System 296
- User Guide 297
- Life Science 300
- Bio-Rad 300
- Laboratories, Inc 300
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