Bio-rad Econo Gradient Pump Kits Manual de usuario

ECONOGRADIENTPUMPEcono Gradient PumpInstruction Manual4006161B.qxd 8/14/2003 8:09 AM Page 1

Front Panel FunctionCancel KeyPressing Cancel will clear an entry that hasbeen input with either the arrow or soft keys.If no entry has been made, the

Front Panel FunctionPurge KeyThis key is used to prime and purge tubinglines without disturbing the primary speedsetting of the pump. When Purge is pr

3.2 REAR PANEL FUNCTIONSFigure 3-2. Rear panel connectors.Display FunctionPower Entry ModuleGrounded 3-pin receptacle for the powercord. Also containe

Display FunctionAux Valve ConnectorThis 8-pin connector controls auxiliaryvalves such as the optional SV-3 Divertervalve or the optional Splitter valv

4.0 TUBING SELECTION AND INSTALLATION4.1 TUBING SELECTIONThe Econo Gradient Pump may be used with most flexible tubinghaving an inner diameter less t

When using tubing other than precut tubing from Bio-Rad, the tubingmust be cut to the following lengths to accommodate prestretch:Tubing LengthTygon,

4.2 TUBING INSTALLATION1. Referring to Figure 4-2, pull the platen cam lever away from thepump head to unlock the platen and slide the platen away fr

3. Insert one end of the tubing into the tubing retaining bracket ofthe pump head. Lightly pull the tubing around the rollers toremove slack. Attach t

5.0 SYSTEM OPERATIONThere are four modes of system operation:• Remote• Setup• Manual• Program, including running the system5.1 REMOTE MODEThe Econo

Screen 1. Flow Rate Selection.Use the soft keys to select either RPM (revolutions per minute) orml/m. This information will be used in writing a prot

SAFETYCaution/WarningDisconnect power to the Econo Gradient Pump beforeservicing. No user-serviceable parts are inside the unit.Refer servicing to Bi

System Operation16Screen 4. Pump Calibration by Measured Flow.Pressing the left or right soft keys will start the pump for the selectedtime. Collect

Screen 6. Selecting Units for Programming.Programming steps can be described in either units of time (inminutes) or volume (in ml).6 Program Steps TI

Screen 9. Biologic System Address Number.The Econo Gradient Pump can be connected to the BioLogic HR orBioLogic Duo-Flow workstation via an Instrumen

Screen 12. Beeper.The beeper can be switched on or off at the user’s discretion. Thebeeper, which beeps on each key press, can be disabled.12 Beep

5.3 MANUAL MODEThe Econo Gradient Pump and attached devices can be controlledusing the pump’s front panel membrane keys and LCD display whilein Manual

5.3.3 Setting Auxiliary (SV-3 or Splitter) Valve ControlControl for an auxiliary valve is selected by pressing the right soft key.This option is avail

Each field may be selected with the corresponding soft key. Thearrow keys are then used to increment or decrement the value.Pressing the Step key or t

5.5 RUNNING A PROGRAMTo start a program (be sure to enter the Program mode by using theMode key), press the Run key. This will start the run. By usin

5.5.1 Holding a RunPressing the Hold key displays the following screen:Holding ___%B CONTINUE Abort When holding, the pump runs indefinitely at the

5.5.4 Power Failure While in Run ModeIn the event of a power failure while a Program is running, the LCDdisplay will indicate:Power Failure! CONTINUE

TABLE OF CONTENTSSafety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iSection 1 Introduction . . . . . . . . . . . . . . . . . . . .

6.0 OPERATION WITH OTHER BIO-RADINSTRUMENTSThe Econo Gradient Pump (EGP) may be used with otherchromatography components (the Model 2110 or Model 212

Model 2128 Fraction Collector (continued)Alternatively, one may use the Model 2128 DiverterValve; it will respond to the program in the Model2128 Frac

Model 2110 Fraction Collector (continued)When a programmed run is started, the EGP willautomatically start the fraction collector. If the SV-3diverter

Model 1327 Chart Recorder (continued)If PAUSE is selected during the program, then thepump and chart recorder will stop.The pump and chart recorder w

BioLogic Duo-Flow SystemThe EGP can be controlled by the BioLogic Duo-FlowSystem provided that the BioLogic System firmwareis version 3.0 or higher.Co

Figure 6-1. System Cabling.Other Instruments31ECONOGRADIENTPUMPMODEL 2110 FRACTION COLLECTORORMODEL 2128 FRACTION COLLECTORMODEL 1327CHART RECORDERCAB

Figure 6-2. System Plumbing.Other Instruments32ECONOGRADIENT PUMPMODEL 2110FRACTIONCOLLECTORMODEL 2128FRACTIONCOLLECTORMODEL EG-1GRADIENTMONITORECONO

7.0 CLEANING AND MAINTENANCEThe Econo Gradient Pump requires very little maintenance to assurereliable operation. The procedures outlined below will

4. The fuse socket in the fuse drawer is removable. Turn the socketsuch that the correct voltage shows through the window.5. Reinsert the fuse drawer

8.0 TROUBLESHOOTINGProblem Possible Cause SolutionPump displays Power outage If you wish to continue a run in“Power Failure!” progress, press the Co

Appendix C Warranty . . . . . . . . . . . . . . . . . . . . . . . 43Appendix D Ordering Information . . . . . . . . . . . . . 44Table of Contentsiv4

Problem Possible Cause SolutionFlow rate not Pump tubing Replace tubing.consistent damaged or worn outPlaten adjustment Adjust the platen by turningin

Problem Possible Cause SolutionCannot Pump must be Insure that pump is stopped.calibrate stopped in order topump calibrate pumpRPM has been Select

9.0 EMBEDDED FUNCTIONSDuring a technical service call, you may be asked to perform taskswhich require you to press combinations of keys. These tasks

APPENDIX ATECHNICAL SPECIFICATIONSNumber of channels 2Flow rate range (per channel) 0.002 - 20 ml/min (depending ontubing diameter)Pump head speed 0.0

Gradient Mixer Range 0-100% BIncrement 1% BValve controlSV-3 Diverter Valve Waste and divert positions.Splitter Valve 0-100%Fraction CollectorsModel 2

APPENDIX BCONNECTION TO OTHER INSTRUMENTSThe I/O connector on the rear panel of the pump is for the operationof the fraction collectors and chart reco

Selected Fraction CollectorPin# Name None 2110 21285 no contact no contact no contact6 ER/PD held LOW held LOW held LOWstarts 1327 starts 1327 starts

APPENDIX C WARRANTYThe Econo Gradient Pump is warranted for one year against defectsin materials and workmanship. If any defects should occur during t

APPENDIX DORDERING INFORMATIONCatalogNumber Product DescriptionEcono Gradient Pump731-9001 Econo Gradient Pump 100/120 V731-9002 Econo Gradient Pump 2

CatalogNumber Product DescriptionTubing and Accessories731-8210 Silicone Tubing, 0.8 mm ID, 0.8 mm wall, 10 m731-8211 Silicone Tubing, 1.6 mm ID, 0.8

1.0 INTRODUCTIONThe Econo Gradient Pump (EGP) is a two-channel, bi-directional,variable speed peristaltic pump for low-pressure chromatography andgen

CatalogNumber Product Description731-9006 Low Pressure, Small Fittings Kit. Contains a subset ofthe fittings found in catalog # 731-8220, including ba

Life ScienceGroupWeb sitewww.bio-rad.com USA(800) 4BIORAD Australia 02 9914 2800 Austria(01) 877 89 01 Canada(905) 712-2771 45 44 52-1000 Finland089

2.0 UNPACKING AND SETTING UP2.1 UNPACKING INSTRUCTIONSCarefully remove the unit from the shipping box, lifting from thehandle on the back of the pump

Your Econo Gradient Pump was carefully tested at the factory andwas shipped in good working order. If any part is missing ordamaged, contact your loca

3.0 PHYSICAL DESCRIPTION AND CONTROLFEATURES3.1 FRONT PANEL FUNCTIONSFigure 3-1. Front Panel Controls and Displays.Description4ECONO GRADIENT PUMPRe

Front Panel Function[LCD; see figure 3-1] LCD DisplayThis display has two rows of 16 characters.Different windows will appear on this displaydepending