Bio-rad ChromLab™ Software Manual de usuario descargar pdf (Pagina 138)

5 | Method Editor

136 | NGC Chromatography Systems and ChromLab Software

Available Range — available ranges for the recipe concentration and pH values

vary depending on the recipe you choose. The ranges provided are

recommendations. You can choose values above the upper end of the range, but

doing so might require other adjustments to ensure accuracy. For most recipes you

can change the buffer concentration by reducing the stock concentration by 1/2 to

1/8 on Conjugate Acid/Base Pair titration and up to 1/4 on Acid or Base titration.

Stock concentration for most recipes is 0.2 M.

Tris and Phosphate are the most commonly used recipes, depending on the type of

chr

omatography being performed.

pH Correction — used to corr

ect the observed pH so it matches the pH set for the

run (Desired pH) in the System Pump dialog box or Method Settings phase. pH

correction uses the difference between the desired pH and observed pH to make

the correction.

Tip: Calibrate the pH probe regularly for accurate pH reading.

Phase Controls and Parameters

The Method Editor includes several standard phases. See Standard Phases on

page 138 for details.

A phase is a method segment composed of a discrete block of steps created to

accomplish a specific task. Each phase comprises contr

ols and parameters specific

to the task it is designed to accomplish.

The controls and parameters used in individual phases are as follows:

 Gradient Segments — determine the length and buffer composition (%B)

for isocratic or gradient steps.

Tip: Each phase runs at a single flow rate. To change the flow rate, select

a dif

ferent phase.

In isocratic steps, initial %B and final %B must be equal. In linear segments,

initial %B and final %B ar

e independent of each other.

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Bio-rad ChromLab™ Software Manual de usuario Pagina 138