Bio-rad ChromLab™ Software Manual de usuario descargar pdf (Pagina 143)

Phase Controls and Parameters

User Guide | 141

Injecting Sample Directly with a Sample Pump

When a sample pump is present in the fluidic scheme and the sample is injected

directly onto the column, you have the option to interrupt the injection if air is

detected. If you select this checkbox and air is detected in the line connected to the

air sensor set to detect end of sample, the system stops the sample pump and the

method continues to the next step.

Fluidic Scheme Includes a Sample Inlet Valve

If your fluidic scheme includes at least one sample inlet valve and you choose to

inject the sample directly onto the column, you have the option to perform a

einjection wash with buffer to flush the sample pump and lines and the loop. If you

select this checkbox, you can choose the port on the sample inlet valve from which

to draw buffer solution. You can define the flow rate and the volume of buffer for the

preinjection wash.

You also have the option to flush the sample pump and related connections after

sample injection. Again, you can choose the port on the sample inlet valve fr

which to draw buf

fer solution and define the flow rate and the volume of the buffer

for the postinjection wash.

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Bio-rad ChromLab™ Software Manual de usuario Pagina 143