Bio-rad ChromLab™ Software Manual de usuario descargar pdf (Pagina 123)

Phase Library Pane

User Guide | 121

Adding a Phase to a Method

To add a phase to a method

 Do one of the following:

 Drag the phase to the appropriate location in the Method Outline pane.

 Double-click the phase to append it after the currently selected phase in

the outline.

Tip: Ensure that the phase is appended after the selected phase, not

at the end of the method outline. If necessary

, reposition the phase by

dragging it into place.

Column Preparation/Activation Before column use, removes the storage

solution and equilibrates the column. By

repeating the phase multiple times, several

preparation solutions can be used one after

another.

Column Performance Test Tests the efficiency of a packed column in

terms of height equivalent to a theor

etical

plate (HETP) and the peak asymmetry

factor (A

System Preparation Before a run, removes storage solution and

fills the system and inlets with buf

fer

solution. This phase pulls from a single

buffer solution each time the phase is used.

Note: System Pr

eparation must be

run as the only type of phase in the

method. You can add several System

Preparation phases to the method if

cycling between multiple solutions is

required.

Create New Phase Adds steps from the Step Library to create

a phase and stor

e it in the custom phase

library.

Table 4. Standard Phases, continued

Phase Explanation

1 2 ... 118 119 120 121 122 123 124 125 126 127 128 ... 299 300

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Bio-rad ChromLab™ Software Manual de usuario Pagina 123