Bio-rad ProteinChip Data Manager Software Manual de usuario descargar pdf (Pagina 19)

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ProteinChip SELDI System: Reader Guide

Multicassette Autoloader (Enterprise Edition)

The ProteinChip SELDI system Enterprise Edition comes with a multicassette

autoloader. It contains cassette loader and unloader assemblies designed to

allow the automated processing of multiple cassettes from a stack. The purpose

of this feature is to allow unattended operation of the reader for signiﬁcant

periods of time. The loader processes the cassettes sequentially, removing each

cassette from an input stack, cycling the designated arrays in the cassette

through the load lock and source chamber, then transferring the processed

cassette to the output unloader before returning to collect the next cassette. This

system is designed for continuous unattended processing of up to 14 cassettes

in one run. A maximum of 14 cassettes can be loaded into the stacker at one

time. The cassette lids must be closed in order for the reader to run.

Overview of Operation

The following instructions are intended to be a brief guide only. For more

information on data acquisition and analysis, please consult the ProteinChip data

manager software manual.

Acquiring control of the reader

The status of the sensors is displayed in the Home tab of the software interface

for the reader (refer to Fig. 12). The sensors should have the following status

before beginning operation:

Enterprise Edition

• Vacuum state — operating

• High voltage — off

• Cassette — present

• Loader cover — closed

• Unloader cover — closed

• High vacuum — <2.7 x 10

-6

Pa.

Control of the reader is obtained by clicking the Start icon.

Personal Edition

• High voltage — off

• Door — closed

• Lock — locked

Control of the reader is obtained by clicking the Start icon.

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Bio-rad ProteinChip Data Manager Software Manual de usuario Pagina 19