Bio-rad Comparative Proteomics Kit I: Protein Profiler Mod Manual de usuario descargar pdf

Comparative Proteomics Kit I:

Protein Profiler Module – Quick

Guide

Lesson 1 Quick Guide

1 Label one 1.5 ml fliptop micro tube for each of

five fish samples. Also label one screwcap micro

tube for each fish sample.

2. Add 250 µl of Bio-Rad Laemmli sample buffer to

each labeled fliptop microtube.

3. Cut a piece of each fish muscle about 0.25 x 0.25

x 0.25 cm

( ) and transfer each piece into a

labeled fliptop micro test tube. Close the lids.

4. Flick the microtubes 15 times to agitate the tissue

in the sample buffer.

5. Incubate for 5 minutes at room temperature.

6. Carefully transfer the buffer by pouring from each

fliptop microtube into a labeled screwcap

microtube. Do not transfer the fish!

7. Heat the fish samples in screwcap microtubes

for 5 minutes at 95°C.

Lesson 2 Quick Guide

1. Set up Mini-PROTEAN 3 gel box and add 1x TGS

electrophoresis buffer to the chamber.

2. Prepare a Ready Gel cassette by cutting

along the black line on the bottom of the

cassette with a razor blade and pulling off

the plastic strip, as indicated on gel

cassette.

3. Remove the comb from the Ready Gel

cassette.

4. Place Ready Gel cassette into the

electrode assembly with the short plate

facing inward. Place a buffer dam or

another Ready Gel cassette on the

opposite side of the electrode assembly,

with notch on buffer dam facing inward.

Student Manual

QUICK GUIDE

1ml

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Pagina 1 - Comparative Proteomics Kit I:

Comparative Proteomics Kit I:Protein Profiler Module – QuickGuideLesson 1 Quick Guide1 Label one 1.5 ml fliptop micro tube for each offive fish sample

Pagina 2 - QUICK GUIDE

5. Slide gel cassette, buffer dam, andelectrode assembly into the clampingframe.6. Press down the electrode assembly whileclosing the two cam levers o

Pagina 3

11. Heat fish samples and actin and myosinstandard to 95°C for 2–5 min.12. Load your gel:Lane Volume Sample1 & 2 empty empty3 5 µl Precision Plus

Pagina 4

45QUICK GUIDEStudent ManualBanana PlugsAnode Banana Plug(Red)Notch onU-Shaped GasketGel SandwichPressure PlateCamsMini TankInnerChamberAssemblyClampin

Pagina 5 - Laboratories, Inc

Life ScienceGroup06-0335 0506 Sig 1205Bulletin 5464 US/EG Rev ABio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Austra

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Bio-rad Comparative Proteomics Kit I: Protein Profiler Mod Manual de usuario

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