Bio-rad ProteinChip Qualification and Calibration Kits Manual de usuario

ProteinChip®System Check KitInstruction ManualCatalog #C70-00081For technical support call your local Bio-Rad office, or in the US, call 1-800-4BIORAD

2.3 System Check Maintenance Procedures 2.3.1 Maintenance Procedure 1 –– High Voltage (HV) Conditioning This procedure helps to decontaminate the su

Note: The detector calibration procedure is intended to standardizeinstrument performance over time. First-time use may alter systemresponse and is no

5. If this is the first calibration performed on the instrument, or if the lastcalibration was performed more than two weeks ago, select two previousl

2.4 System Check Tests2.4.1 Test 1 –– Detector Sensitivity This test uses the ProteinChip detector qualification array to measurethe signal-to-noise

6. Plot the peaks with noise set to 12,000 Da:a. Select all 8 spectra.b. Click on the data analysis icon . The Analysis Settingsdialog opens.c. Open

7. Plot all 8 spectra and select the IgG peak using the peak selectiontool.Note: Spots with high concentrations (spots A, C, E, and G) alternatewith t

2.4.2 Test 2 — Mass Drift and Resolution 5.96 kD This test uses the ProteinChip peptide standard array to measure themass drift and resolution of ins

6. Run the same protocol on partition “n” of 4 for all 8 spots. Recordthe partition number on the usage chart provided with the productpackaging.7. Ma

8. Plot the peaks with the filtering option turned off:a. Select all 8 spectra. (Do not select the first laser optimizationspectrum.)b. Click the anal

9. Plot all 8 spectra. Using the peak selection tool, select the insulin peak(5.963 kD) in all spectra.10. Export the data according to the instructio

NoticesNo part of this publication may be reproduced or transmitted in any formor by any means, electronic or mechanical, including photocopy, recordi

Appendix AData Export ProcedureUse this procedure to export the data obtained during testing to theMicrosoft Excel spreadsheet provided with the kit (

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Table of ContentsChapter 1 Introduction ...11.1 Product and Protocol Description ...

Chapter 1Introduction1.1 Product and Protocol DescriptionThe ProteinChip system check kit provides tools for performing twomaintenance procedures (de

Table 1. Summary of procedures and tests.CCoonnssuummaabbllee EEssttiimmaatteedd TTiimmee TTiittllee UUsseedd RReeqquuiirreedd DDeessccrriippttiio

Table 1. Summary of procedures and tests (continued).CCoonnssuummaabbllee EEssttiimmaatteedd TTiimmee TTeesstt UUsseedd RReeqquuiirreedd DDeessccr

Chapter 2Use of the ProteinChip System Check Kit2.1 OverviewThis chapter outlines the protocols used to perform the procedures andtests that comprise

• When running protocols, you specify how many sections (partitions)the spot is divided into for sampling. You also specify which of thesepartitions s

Step 3Set Up theHigh Voltage (HV)Conditioning Routine61. Insert a blank ProteinChip array into the reader. If blank arrays are notavailable, use expir