Bio-rad BioLogic LP System Manual de usuario Pagina 31
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4.2 ADJUSTING THE PLATEN AND PLUMBING THE PERISTALTIC PUMP
The peristaltic pump requires the following tubing lengths:
• PharMed 179 ±1.5mm
• Silicone 171±1.5mm
Note: PharMed is preferred. (Tygon should not be used in the pump head because it fatigues
rapidly.) If you are not using pre-cut tubing, cut the tubing to the lengths indicated above.
1. Proper adjustment of platen pressure increases flow stability, minimizes flow pulsation, and prolongs the
life of the tubing. To adjust platen pressure, use the platen adjustment screw. First turn the platen
adjust screw counterclockwise until it stops. Then turn the screw clockwise according to the table
below:
Number of Turns
T
ubing ID From Fully Open
0.8 mm (1/32”) 5
1.6 mm (1/16”) 4
3.2 mm (1/8”) 3
Note: Overtightening the platen reduces flow rate and shortens tubing life. A loose platen will cause the
flow rate to decrease as the backpressure increases.
Figure 13. Plumbing the BioLogic LP Controller’s Pump
24
System Plumbing
1
Alarm
23
456
78
0
9
C.
PLATEN ADJUST
SCREW
LOCK-RING
TUBING
LUER FITTING
SEE DETAIL
- BIOLOGIC LP 1
- CHROMATOGRAPHY SYSTEM 1
- TABLE OF CONTENTS 2
- GETTING STARTED QUICKLY 6
- 1.0 INTRODUCTION 8
- 1.2 FEATURES 9
- 1.3 UNPACKING 10
- Table 1. (continued) 11
- 2.0 DESCRIPTION OF COMPONENTS 13
- Table 3 14
- Table 3. (continued) 15
- 2.3 UV DETECTOR 17
- 2.4 CONDUCTIVITY DETECTOR 18
- 2.5 BIO-RAD SYSTEM OPTIONS 19
- Threshold 20
- Next tube 2 20
- Draining 20
- 3.0 SYSTEM CONNECTIONS 24
- Figure 10. Rack Assembly 25
- System Connections 27
- 4.0 SYSTEM PLUMBING 29
- Figure 12. Luer Fittings 30
- PLATEN ADJUST 31
- LOCK-RING 31
- LUER FITTING 31
- 4.3 PLUMBING THE SYSTEM 33
- Figure 14. System Plumbing 34
- 4.4 PURGING THE SYSTEM 37
- 5.0 SYSTEM OPERATION 38
- Table 5. (continued) 39
- Front Panel Controls 39
- 5.2 MANUAL MODE 41
- System Operation 42
- Table 6. (continued) 43
- Manual Mode Operation: Pump 43
- Table 6. (continued) 44
- Table 6 45
- Table 7 45
- Table 7. (continued) 46
- Table 8 48
- Table 9 49
- Table 9. (continued) 50
- Table 10 51
- Table 10. (continued) 52
- Table 11 53
- Table 11. (continued) 54
- Table 12 55
- 5.3 PROGRAMMING MODE 56
- Table 13 58
- Table 13. (continued) 59
- Figure 22. List of Methods 59
- 5.3.2 Creating a New Method 60
- Table 15 63
- Table 15. (continued 64
- Table 16 65
- Program Mode’s Pump Table 65
- Table 16. (continued) 66
- Table 17 69
- Table 17. (continued) 70
- Table 18 71
- Table 19 72
- Table 20 74
- Table 20. (continued) 75
- Table 21 77
- Table 21. (continued) 78
- Table 22 80
- Program Mode’s Alarm Table 80
- Table 22. (continued) 81
- 5.3.7 Entering Method Names 82
- 5.4 RUN MODE 83
- 5.4.1 Starting a Run 83
- Table 24. (continued) 84
- Run Mode: Starting a Run 84
- 5.4.2 Run in Progress 86
- Table 24 87
- Table 25 88
- Run Mode: Run Hold 88
- Table 26 89
- Run Mode: Run Pause 89
- 6.1 CLEANING AND STORAGE 92
- 6.2 PUMP CALIBRATION 92
- 6.2.1 Nominal Calibration 93
- 6.2.2 User Calibration 93
- Figure 32. UV Optics Module 96
- 6.6 TROUBLESHOOTING 97
- APPENDIX A. SPECIFICATIONS 99
- WARRANTY INFORMATION 101
- ORDERING INFORMATION 102
- 4006072 Rev D 106
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