Bio-rad ZOE™ Fluorescent Cell Imager Manual de usuario descargar pdf (Pagina 17)

ZOE Fluorescent Cell Imager Instruction Manual | 9

Focus position indicator on the left side of the touch screen shows the Z axis position number.

The number is updated during focusing (Figure 4). Knowing the approximate Z number for the

tissue culture vessels (Table 2) you use can speed up focusing.

Table 2. Z axis position range of tissue culture vessels.

Vessel Type Z Axis Position

Microscope slide 720 (± 100)

6-well plate 2,000 (± 100)

12-well plate 2,100 (± 100)

24-well plate 1,900 (± 100)

48-well plate 2,000 (± 100)

96-well plate 3,500 (± 200)

T25 ﬂask 1,800 (± 200)

T75 ﬂask 2,500 (± 200)

T225 tissue culture ﬂask 3,200 (± 200)

Small petri dish 1,200 (± 200)

Large petri dish 1,100 (± 200)

Optimizing Imaging Parameters in Brightﬁeld Channel

Use the Edit menu to adjust these precapture parameters (Figure 5): Gain, LED Intensity, and

Contrast values; Exposure time (msec); and quadrant illumination. Drag the slider bar to adjust a

parameter. Tap the – and + controls at either end of each slider bar for ﬁne adjustments.

When searching for sample — increase Gain value for brighter signal; reduce Exposure

time for faster frame rate

Once the sample is identiﬁed and ready to be captured — lower Gain value to reduce

background noise; increase Exposure time to regain signal intensity

To further increase brightness — start by increasing LED Intensity value; follow by

increasing Exposure time if needed

To reduce nonspeciﬁc background signal — increase Contrast value

Fig. 5. Edit controls in the brightﬁeld channel.

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Bio-rad ZOE™ Fluorescent Cell Imager Manual de usuario Pagina 17