Bio-rad VINEO™ Brettanomytest PCR Kit Manual de usuario descargar pdf (Pagina 11)

APPENDIX A - PCR AMPLIFICATION REAGENT MIXTURE PREPARATION TABLE

Use this table to determine the required quantities of fluorescent probes and amplification

solution to prepare the PCR reagent mixture. Calculation of the number of samples

must include the 2 positive Qs PCR controls and the negative control.

Number

of samples

Fluorescent

probes (μL)

(Pink stopper)

Ampliﬁcation

solution (μL)

(White stopper)

Number

of samples

Fluorescent

probes (μL)

(Pink stopper)

Ampliﬁcation

solution (μL)

(White stopper)

1 5 40 49 265 2117

2 11 86 50 270 2160

3 16 130 51 275 2203

4 22 173 52 281 2246

5 27 216 53 286 2290

6 32 259 54 292 2333

7 38 302 55 297 2376

8 43 346 56 302 2419

9 49 389 57 308 2462

10 54 432 58 313 2506

11 59 475 59 319 2549

12 65 518 60 324 2592

13 70 562 61 329 2635

14 76 605 62 335 2678

15 81 648 63 340 2722

16 86 691 64 346 2765

17 92 734 65 351 2808

18 97 778 66 356 2851

19 103 821 67 362 2894

20 108 864 68 367 2938

21 113 907 69 373 2981

22 119 950 70 378 3024

23 124 994 71 383 3067

24 130 1037 72 389 3110

25 135 1080 73 394 3154

26 140 1123 74 400 3197

27 146 1166 75 405 3240

28 151 1210 76 410 3283

29 157 1253 77 416 3326

30 162 1296 78 421 3370

31 167 1339 79 427 3413

32 173 1382 80 432 3456

33 178 1426 81 437 3499

34 184 1469 82 443 3542

35 189 1512 83 448 3586

36 194 1555 84 454 3629

37 200 1598 85 459 3672

38 205 1642 86 464 3715

39 211 1685 87 470 3758

40 216 1728 88 475 3802

41 221 1771 89 481 3845

42 227 1814 90 486 3888

43 232 1858 91 491 3931

44 238 1901 92 497 3974

45 243 1944 93 502 4018

46 248 1987 94 508 4061

47 254 2030 95 513 4104

48 259 2074 96 518 4147

1 2 ... 6 7 8 9 10 11 12 13 14 15 16 ... 55 56

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Bio-rad VINEO™ Brettanomytest PCR Kit Manual de usuario Pagina 11

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