Bio-rad SmartSpec Plus Spectrophotometer Manual de usuario descargar pdf (Pagina 53)

Problem Cause Solution

RS232 serial port/no Incomplete system 1. Power down unit/Restart unit.

response initialization during power up.

PC setup incorrect/wrong 2. Check spreadsheet program

instruct mode. setup.

Cable's not fully connected/ 3. Check all connections.

wrong adaptor.

Serial Port not selected in 4. Enter Setup function and

Setup ensure that the serial port is

activated.

Cuvette does not fit in Cuvette wrong size. 1. Chamber accommodates

cuvette chamber cuvettes up to 12.5 mm width.

Cuvette spring in wrong 2. Re-insert spring to correct

location. location.

Cuvette spring missing. 3. Obtain new cuvette spring.

Negative values/no No sample. 1. Add Sample to solution

absorbancy

Cuvette light path 2. Check cuvette window

orientation incorrect. orientation.

Wrong wavelength setting. 3. Check assay wavelength

requirement.

May have blanked the 4. Reblank the instrument using

instrument using sample buffer or water.

instead of blank solution.

Cuvette window "Z" 5. Use only 8.5 mm "Z"

height incompatible with dimension cuvettes.

cuvette chamber.

(See Section 11.4.)

Fluctuations Interference in light path. 1. Check that there are no

scratches on the cuvette.

2. Check that there are no

particles floating in the

cuvette.

3. Wipe the cuvette walls with

lint-free tissue.

4. Make sure the sample is in

the recommended working

range i.e. 0.1–1.5 AU.

5. Clean cuvette chamber as

described in Section 9.1).

Section 9

Maintenance

9.1 Cleaning Up a Fluid Spill

In case of a spill in the cuvette chamber, clean up the spill using the following proce-

dure:

1. Turn the instrument off and unplug the power cord.

2. Use a damp coton swab to clean the cuvette chamber.

3. Use a cotton swab to dry the cuvette chamber.

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Bio-rad SmartSpec Plus Spectrophotometer Manual de usuario Pagina 53

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