Bio-rad Quantum Prep Plasmid Kits Manual de usuario descargar pdf (Pagina 7)

1.3 Storage and Stability

All components are stable for 12 months from the date of

purchase when stored at room temperature and used as described

in this manual.

Section 2

Protocol

2.1 Recommendations for Best Results

• Thelysisandneutralizationsolutionsmayexhibitsaltprecipitation

due to cold temperatures. The product will not perform optimally

if the salt precipitates out of the solution. If precipitation is

observed upon receipt, warm the bottles to 37°C with

occasional mixing until redissolved. Store at room temperature.

• The1.5mlmicrocentrifugecollectiontubessuppliedwiththis

kit will accomodate the spin filters for elution of purified

plasmid. The spin filters will also fit in most commonly available

1.5 ml microcentrifuge tubes.

• ToimprovethetotalyieldofDNA,elutewithtwicethe

recommended volume (200 µl) of deionized H

O or TE (10 mM

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Bio-rad Quantum Prep Plasmid Kits Manual de usuario Pagina 7