Bio-rad Model 680 Microplate Reader Accessories Manual de usuario Pagina 1

Model 680 Microplate Reader Instruction Manual Catalog Numbers 168-1000 168-1001 168-1002 168-1003

6 Section 3. Instrument Set-up 3.1 Initial Start-up 1 Place the instrument on a clean, sturdy table or bench. It is important to keep the instrum

7 Section 4. Operation 4.1 Instrument Overview The Model 680 Microplate Reader has built-in software that allows the user to set the plate reading

8 Protocol Security Filters Date Set Lab name Cutoff Report Limit STDs Mode Mapping Kit name Not Use Constant Control FormulaRatio Ranged Cutoff Sing

9 .Func key to switch from [ABC] to [abc] to [sym] key to move cursor .Func key to switch from [ABC] to [abc] to [sym] key to move cursor .Fu

10 4.2 Functions Overview 4.2.1 Calculations The Microplate Reader uses Beer's Law to calculate the absorbance value of each well. Beer's

11 in this memory. The first time the instrument is turned on, or after a battery failure, the following default information will be held in the mem

12 Blank mean = X/n S.D. = [{X^2 – n*(Blank mean)^2}/{n-1}]^1/2 Where: S.D. = Standard deviation X = Sum total of the raw absorbance for each blank X

13 Upper cutoff absorbance = Positive absorbance + ((Gray zone/100) * Positive absorbance) Lower cutoff absorbance = Positive absorbance – ((Gray zo

14 The result absorbance value of the formula calculation and the gray zone value that is entered by the user are used for the cutoff. The upper and

15 i. 5P Logistic Rodbard ii. 4P Logistic Rodbard iii. 5P Logistic Cook-Wilkenson iv. 4P Logistic Cook-Wilkenson v. 5P Exponential vi. Sigmoid Logist

i Warranty Statement This warranty may vary outside of the continental United States. Contact your local Bio-Rad office for exact terms of your war

16 Negative cutoff = (Negative Control average + 0.210) * 0.900 TeSeE eq: Positive cutoff = Negative Control average + 0.210 Negative cutoff = Negat

17 4.2.7 Printer The Model 680 Microplate Reader has a built-in printer interface connector (25 pin D-sub) or an optional factory built-in printer. T

18 4.3 Detailed Operation 4.3.1. Initial Start-up 01:Kinetic-1 M405(1)R605(6)30readShake:999s,Mid,Every38.2/40

19 4.3.2. Configuring the system Note: To return to the previous screen, use the back/left arrow key.

20 1:400nm 5:650nm 2:450nm 6:...nm 3:475nm 7:...nm 4:650nm 8:...nm Filter Day/Month/Ye

21 4.3.3. Printing Reports and Protocol information Print menu » Reports •Protocol PRINT MENU Print Menu This menu

22 4.3.4. Memory Recall operation 01 20/05/00 14:00 02»*21/05/00 17:00 03 20/05/00 18:00 ↓04»

23 4.3.5. Editing Kinetic protocol Kinetic Protocol Menu Select “Protocol” in the Edit Menu and p

24 Kit name for #01 »G.A.P. TEST IgG« ↑ [ABC] Kit name Calculation Mapping As

25 4.3.6. Checkmark value setting (by Administrator only) » Serial #/Report •Linearity ref. •Max

ii Regulatory Notices Electro Magnetic Compatibility: This instrument is designed to meet the Class-A emissions and immunity levels of the EN61326

26 4.3.7. Editing End Point Protocol Upper Limit= 2.000 Lower Limit= 0.000 input range: 0–3.500 U

27 4.3.8. Cutoff setting procedure Cutoff setting Menu This menu is entered by choosing "Cutoff" in the End Point Protocol Menu. It is us

28 b. Cutoff Control setting Selection of Single/Ranged Cutoff Control This menu screen selects Single or Ranged cutoff type. The cutoff type marked

29 c. Cutoff Formula setting Selection of Formula This menu screen selects a formula for cutoff calculation. The formula marked with “[ ]” is the c

30 d. Cutoff Ratio setting Ratio to Calibrator In this screen, there are three items for setting the ratio to calibrator. The Up and Down arrow key

31 4.3.9. Standard setting procedure Standard curve setting Menu This menu screen is entered by choosing "STDs" in the End Point Protocol

32 b. Curve fit setting Curve fit In this screen, there are two items for setting the curve fit. The Up and Down arrow key moves the cursor to the

33 c. Recall STD curve setting Recall Setting of Standard Curve In this screen, there are two items for setting the Recall STD curve as below. The U

34 4.3.10. Mode setting procedure Mode setting Menu This menu screen is entered by choosing the "Mode" in the End Point Protocol Menu sc

35 d. Incubation Incubation In this screen, the user switches on/off the Incubator and sets the incubation temperature. The temperature setting rang

iii Table of Contents Section 1. ...Introduction ...

36 4.3.11. Plate Map setting procedure Select Mapping mode Menu This menu screen is entered by choosing the "Mapping" in the End Point Pr

37 Acceptable range of number for each well type: 1. Blank and Empty wells are not acceptable for any number. 2. Sample and Standard wells: 00

38 Warning and Error of Automatic Mapping: Warning screen: If the total number of mapping wells exceeds 96 wells, a warning message in the left figu

39 4.4 Quick Guide – Reading a plate 1. Turn on the instrument. Allow about 15 seconds for the self-diagnosis. Allow the instrument to warm up fo

40 4.5 Language choice The Model 680 Microplate Reader has two versions, English and Chinese, in the on-board software. And it allows the user to ch

41 (5). Retrieval function for plate data The Chinese version does not support this feature. The URPD (Upload Raw Plate Data) and DRPD (Download Raw

42 Section 5. Instrument service by the user Caution: Electrical shock hazardous! Always disconnect the instrument from the AC powe

43 Section 6. Troubleshooting and Error Messages The Model 680 Microplate Reader constantly monitors several functions and will display appropriat

44 7. LCD displays “Filter not installed”. Enter the Edit Menu by pressing Edit key, and set up the filters. 8. Wells have color, but absorbance

45 Section 7. Specifications 7.1 Instrument Specifications Operating panel Membrane pad with 4 arrow keys, 7 function keys and 11 numeral keys Di

1 Section 1. Introduction The Model 680 Microplate Reader is an eight-channel, vertical pathlength photometer that measures the absorbance of the c

46 Accuracy ±1.0% or 0.010 from 0 to 3.0 OD at 490nm Linearity ±1.0% from 0 to 2.0 OD, ±2.0% from 0 to 3.0 OD Reproducibility 1.0% or 0.0

47 7.2 Interface Specifications The following specification describes the syntax and language required for computer control of the reader. 1. SI

48 7.3 Command Language for Model 680 Microplate Reader 7.3.1 Command Language for Remote control mode 1-1. Syntax SYNTAX: Command: <devic

49 1-2-6 RM (Reset maintenance report) <command>: "RM" Reset maintenance report values. <arguments>: none <response>: &qu

50 1-2-11. RWELL (Read a plate well) <command>: “RWELL” Read a plate well <arguments>: Single reading:<cl><space><rw>&

51 1-2-14. UPLD (Upload the protocol data) <command>: "UPLD" Upload the protocol data <arguments>: <up load protocol numbe

52 1-2-20. SSN (Set Serial Number) <command>: "SSN" Set Serial number <arguments>: <Serial number> <Serial number>

53 1-3. Absorbance Data 1-3-1. Abs data <abs.data>: <header><date time><reading info><Mes. data><cr>[<Ref.

54 1-4. Protocol data All protocol data is ASCII code data. Transmit format: <“,”(0x2c)><protocol data 1>< “,”><protocol dat

55 (data length max: 4 characters(include decimal point)) 23 Cutoff negative const value “.001 to 9999” (data length max: 4 characters(include decima

2 1.1 Description and accessories for Model 680 Microplate Reader Catalog Number Description 168-1000 Model 680 microplate reader 168-1001 Model

56 [7]: Difference subtraction 0:Even-Odd (column) 1:Odd-Even(column) 2:Even-Odd(row) 3:Odd-Even(row) [8]: Concentration (“ “(0x20) data delimits pri

57 1-5. “TSE” and “TSE eq” Assay Parameter Format “TSE” and “TSE eq” parameter is same format. All parameter data is ASCII code data. Transmit fo

58 1-6. Upload Raw Data Format 1-6-1. Header Type Format of the Upload Raw Plate Data <upload raw plate data>: <memory #><data exist

59 1-6-2-2. Full Data Type Format for Kinetic <upload raw plate data>: <memory #><data exist><date time><plate #><

60 1-7. Download Raw Plate Data Format All protocol data is ASCII code data. Transmit format: <“,”(0x2c)><plate datum 1><“,”><p

61 Abs. Data Sample of <Mes. data> and <Ref. data> " 0.101 0.102 0.103 0.104 0.105 0.106 0.107 0.108 0.109 0.110 0.111 0.112"&l

62 (2) Reading number= "1" to "30" 5 Plate reading date <year>”/”<month>“/”<day><space><hour>”:”&l

63 7.3.2 Error Codes Error code Error Cause Recovery 0000 No error 8071 Invalid Command The received data is not a command for the instrument 8

64 4000155 Rev F

3 Section 2. Product Description 2.1 Contents of Shipping Carton The shipping carton contains the following items: • Model 680 Microplate Read

4 2.2 External Features 1. Release latch for rear cover 4. Rear cover 2. Liquid crystal display (LCD) 5. Printer slot (wh

5 2.3 Membrane Keypad 1. Main Returns to MAIN SCREEN. 2. Start/Stop Initiates plate reading using current active pr