Bio-rad Microplate Manager Software Manual de usuario descargar pdf (Pagina 82)

Microplate Manager User Guide

• Normalization Report: Similar to the Limit Report, except that the readings are

displayed as percentages relative to the limits.

• Standard Curve Report: Displays the interpolation curve defined by your

standards, as well as associated statistical analyses. Five different regression

methods (linear, quadratic, cubic, cubic-spline, and four-parameter logistic) are

available.

• Unknown Concentrations Report: Unknown concentrations are computed

based on the standards and regression method you have chosen.

The following reports are specific to endpoint and multiple-plate protocols

(absorbance data):

• Raw Data Report: Displays the raw absorbance values for the microplate wells.

• Absorbance Report: Subtracts the mean absorbance of the blank wells from the

raw absorbance of each well.

The following reports are specific to kinetic protocols (velocity data):

• Velocity Report: Shows the velocity of each well, calculated from the raw data

as specified in the Analysis Options dialog box.

• Adjusted Velocity Report: Subtracts the mean velocity of the blank wells from

the raw velocity of each well.

• Kinetic Correlation Coefficient Report: Shows the correlation coefficient of

the linear fit for each well.

• Kinetic Standard Errors Report: Shows the standard error of the linear fit for

each well.

• Kinetic Plots Report: Shows the time evolution graph of each well.

• Kinetic Zoom Plot Report: Shows the kinetic plots data for a selected well.

Shows the fitted equation for the linear regression and its standard statistics.

From within a protocol dialog box, you can select the reports to be automatically

displayed after a protocol is run. See section 5.1.6 for instructions.

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Bio-rad Microplate Manager Software Manual de usuario Pagina 82