Bio-rad Ligation and Transformation Module Manual de usuario descargar pdf (Pagina 10)

Kit Inventory Checklist

This section lists equipment and reagents necessary to perform the ligation

and transformation protocol in your classroom or teaching laboratory. Each

kit contains sufficient materials for 12 student workstations, 12 ligation

reactions, and 24 transformations. We recommend that students are

teamed up – two to four students per workstation. Please use the checklist

below to confirm inventory.

Kit Components Number/Kit (✔)

T4 DNA ligase, 10 µl 1

❒

Ligation reaction buffer (2x concentration), 100 µl 1 ❒

Proofreading polymerase, 10 µl 1 ❒

pJet1.2 blunted vector, 10 µl 1 ❒

Sterile water, 1 ml 1 ❒

Bgl II restriction enzyme, 50 µl 1 ❒

10x Bgl II reaction buffer, 1 ml 1 ❒

Isopropyl b-D-1-thiogalactopyranoside (IPTG), 1 M, 0.1 ml 1 ❒

Transformation reagent A, 1.25 ml 4 ❒

Transformation reagent B, 1.25 ml 4 ❒

C-growth medium, 30 ml 1 ❒

Microcentrifuge tubes, clear, 1.5 ml 30 ❒

Microcentrifuge tubes, multicolor, 2.0 ml 120 ❒

1 2 ... 5 6 7 8 9 10 11 12 13 14 15 ... 59 60

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Bio-rad Ligation and Transformation Module Manual de usuario Pagina 10