Bio-rad Comparative Proteomics Kit II: Western Blot Module Manual de usuario descargar pdf (Pagina 6)

QUICK GUIDE

Quick Guide

6. Set up the Mini Trans-Blot module with the

black side of the cassette next to the black

side of the Mini Trans-Blot module. Add a

frozen Bio-Ice module and fill with blotting

buffer up to the white clip.

7. Place lid on tank, matching the power

cords red-to-red and black-to-black, then

blot at 20 V for 2.5 hours.

8. At this point the blots can be stored in the

tanks submerged in blotting buffer at room

temperature overnight or the sandwiches

dismantled and the blots placed in blocker

overnight at 4°C.

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Bio-rad Comparative Proteomics Kit II: Western Blot Module Manual de usuario Pagina 6