Bio-rad Comparative Proteomics Kit II: Western Blot Module Manual de usuario descargar pdf (Pagina 3)

Quick Guide

QUICK GUIDE

11. Heat fish samples and actin and myosin

standard to 95°C for 2–5 min.

12. Load your gel:

Lane Volume Sample

1 & 2 empty Empty

3 5 µl Precision Plus Protein

Kaleidoscope

prestained standards

4 5 µl fish sample 1

5 5 µl fish sample 2

6 5 µl fish sample 3

7 5 µl fish sample 4

8 5 µl fish sample 5

9 5 µl actin and mysin standard

(AM)

10 empty empty

13. Electrophorese for 30 minutes at 200 V in 1x

TGS gel running buffer.

14. Proceed directly to Lesson 3, continue to step 15

of Lesson 2 to stain gels or store gels overnight

at 4°C.

15. If the gels are to be stained, save 50 ml of 1x TGS

gel running buffer.

16. Remove gel from cassette and transfer gel to a

container with 25 ml Bio-Safe Coomassie

stain/per gel and stain gel for 1 hour, with gentle

shaking for best results.

17. Discard stain and destain gels in a large

volume of water for at least 30 minutes to

overnight, changing the water at least once.

Blue-stained bands will be visible on a clear gel

after destaining.

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Bio-rad Comparative Proteomics Kit II: Western Blot Module Manual de usuario Pagina 3