Bio-rad Aurum™ Total RNA Mini Kit Manual de usuario descargar pdf (Pagina 5)

Section 1

Introduction

The Aurum™ total RNA mini kit purifies total RNA samples rapidly from

mammalian cell cultures, bacteria, and yeast (Saccharomyces cerevisiae), as

well as animal and plant tissue. Total RNA samples prepared using the Aurum

total RNA mini kit are suitable for use in a variety of downstream applications,

including reverse transcription-PCR (RT-PCR), real-time PCR, and northern

blots. A DNase l digest during the purification effectively removes genomic

DNA contamination from the preparation, eliminating the need for separate

DNase digests. All solutions and binding columns in the kit are RNase-free,

ensuring the integrity of the isolated total RNA. The Aurum total RNA mini kit

may be used in a spin or vacuum format using the Aurum vacuum manifold

(catalog # 732-6470).

Section 2

Kit Components

The Aurum™ total RNA mini kit contains the following components:

Components* Quantity/Amount

RNA binding columns 50

Capless wash tubes, 2 ml 50

Capped microcentrifuge tubes, 1.5 ml 50

Capped microcentrifuge tubes, 2.0 ml 100

DNase I (lyophilized) 1 vial

Lysis solution 50 ml

Low stringency wash solution

(5x concentrate) 20 ml

High stringency wash solution 40 ml

Elution solution 20 ml

DNase dilution solution 20 ml

*There may be reagent remaining in some bottles.

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Bio-rad Aurum™ Total RNA Mini Kit Manual de usuario Pagina 5