Bio-rad Aurum™ Total RNA Mini Kit Manual de usuario descargar pdf (Pagina 14)

Manifold Wash Setup (Figure 2)

1. Insert the CAP (luer ends up) into the depression in the vacuum manifold

top. Ensure that the CAP rests evenly on the gasket.

2. Insert the luer ends of the desired columns into the available luer fittings,

ensuring a tight fit.

3. Close the unused luer fittings with the caps provided. Close caps by

rotating clockwise until light resistance is encountered. Excessive

tightening of a cap may cause the luer fitting to dislodge when the cap is

removed.

4. The manifold is now ready for column processing according to the

vacuum protocol of the appropriate column purification kit.

5. When ready to elute, proceed with the appropriate spin elution step as

recommended by the protocol.

6. After finishing the elution, rinse the CAP and Aurum vacuum manifold

with water and air dry or wipe with paper towels.

Fig. 2. Manifold setup for column processing.

CAP

Manifold top

Manifold base

4110133B:4110133A.qxd 1/16/2009 2:35 PM Page 10

1 2 ... 9 10 11 12 13 14 15 16 17 18 19 ... 27 28

Sin comentarios

Bio-rad Aurum™ Total RNA Mini Kit Manual de usuario Pagina 14