Bio-rad Aurum™ Total RNA 96 Kit Manual de usuario descargar pdf (Pagina 22)

Problem Possible Cause Recommended

Solution

Total RNA prep Incorrect use of wash Add the appropriate

performs poorly in  solutions volumeof95–100%

downstream ethanol to the low

applications stringency wash

solution before initial

use

RNA is degraded See problem “RNA

degradation”

Ethanol contamination in Add 1–3 min to the

prep (eluate volumes purge time after the final

  >60µl) washstep

    BlotRNAbindingplate

drip directors with paper

towels; ensure that the

plate underside is dry

Inefficient drying Dry for a full 4 min and

ensure no droplets of

wash buffer remain on

the walls or the bottom

of the plate

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Bio-rad Aurum™ Total RNA 96 Kit Manual de usuario Pagina 22