Bio-rad Aurum™ Total RNA 96 Kit Manual de usuario descargar pdf (Pagina 21)

Problem Possible Cause Recommended Solution

Clogging of RNA Excessive amount of Reduce volume of

binding plate starting material per well culture used

Incomplete mixing of lysis Mix RNA lysis solution

and alcohol solutions and alcohol thoroughly

by pipetting up and

down

Incomplete Pipette lysate up and

homogenization of cell down further to reduce

lysate lysate viscosity

Incomplete digestion with Increase duration of

lysozyme or lyticase lysozyme or lyticase

digestion; use fresh

enzyme

Low RNA yield Low amount of starting Increase starting material

material amount up to the

maximum indicated for

the specific starting

material type

Incorrect use of wash Add the appropriate

  solutions volumeof95–100%

ethanol to the low

stringency wash solution

before initial use

Incorrect preparation Use only the DNase

of DNase dilution dilution solution provided

in the kit to dilute the

DNase I

Low sample eluate See problem “Low or

volume highly variable eluate

volumes among wells”

Inefficient elution Preheat the elution

solution to 70°C in water

bath prior to the elution

step

Incomplete lysis Pipet lysate up and down

further to facilitate lysis

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Bio-rad Aurum™ Total RNA 96 Kit Manual de usuario Pagina 21