Bio-rad Profinity eXact™ Purification and Tag Cleavage Con Manual de usuario descargar pdf (Pagina 3)

Section 1

Introduction

Bio-Scale Mini cartridges are convenient, disposable,

prepacked chromatography cartridges. Patented*

column design assures leak-free operation with any

low pressure chromatography system. Bio-Scale

Mini cartridges are available for a variety of

chromatographic techniques such as desalting, ion

exchange, hydrophobic interaction, and affinity

chromatography.

Bio-Scale Mini Profinity eXact cartridges are packed

with Profinity eXact (EX

act Affinity Cleavage T

echnology)

purification resin. This agarose-based affinity

chromatography resin utilizes an immobilized, modified

protease that selectively binds the fusion protein

and cleaves the affinity tag on-column under

controlled conditions, releasing the purified target

protein which contains only its native amino acid

sequence. This innovative resin technology improves

the efficiency of recombinant protein purification, and

is the only affinity chromatography platform that

completes the purification and tag removal process in

a single step

*U.S. patent 7,208,087

Section 9 Scaling Up........................................23

Section 10 Regenerating, Cleaning,

and Storage .....................................23

Section 11 Troubleshooting Guide......................26

Section 12 Ordering Information.........................28

Section 13 References .......................................29

Section 14 Legal Notices ...................................29

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Bio-rad Profinity eXact™ Purification and Tag Cleavage Con Manual de usuario Pagina 3