Bio-rad Microplate Manager Software Manual de usuario Pagina 55

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Analyzing Data Using Cutoffs
47
Reference can be made to standard or sample IDs that have been defined in the
Template setup
Standards: enter ! followed by concentration
Unknowns: enter identifier
Blanks: enter #
In all Cutoff equations, Microplate Manager 6.0 uses the averaged value of that
group.
Examples of valid expressions:
U01
(U01+.1)/2
(!100-!10)*2
Cutoff Examples for Assays with Controls
Positive & Negative Controls – positive (POS) & negative (NEG) control well(s)
defined on template
Low Cutoff: NEG
High Cutoff: POS
% B/Bo – non-specific binding blank (NSB) & maximum or Bo well(s) (MAX)
defined on template
Low Cutoff: NSB
High Cutoff: MAX
% Calibrator –blank (#) & calibrator (CALB) well(s) defined on template
Low Cutoff: #
High Cutoff: CALB
% Control - non-specific binding blank (NSB) & control well(s) (CNTRL) defined
on template
Low Cutoff: NSB
High Cutoff: CNTRL
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